Construct: shRNA TRCN0000110422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011674.2-510s1c1
DNA Barcode:
CCCAATGATATGTGTGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt8a (22239)
Gene Description:
UDP galactosyltransferase 8A
Transcript:
RefSeq NM_011674.2 (NON-CURRENT)
Match location:
Position 510 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGATATGTGTGGATTT
Hairpin Sequence:
5'-CCGG-CCCAATGATATGTGTGGATTT-CTCGAG-AAATCCACACATATCATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGATATGTGTGGATTTCTCGAGAAATCCACACATATCATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGATATGTGTGGATTTCTCGAGAAATCCACACATATCATTGGG-3'

Other clones with same target sequence:

(none)