Construct: shRNA TRCN0000110429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153598.1-607s1c1
DNA Barcode:
TGCCTGTTGTTATGTCAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt2b34 (100727)
Gene Description:
UDP glucuronosyltransferase 2 family, polypeptide B34
Transcript:
RefSeq NM_153598.1 (NON-CURRENT)
Match location:
Position 607 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCTGTTGTTATGTCAGAAT
Hairpin Sequence:
5'-CCGG-TGCCTGTTGTTATGTCAGAAT-CTCGAG-ATTCTGACATAACAACAGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCTGTTGTTATGTCAGAATCTCGAGATTCTGACATAACAACAGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCTGTTGTTATGTCAGAATCTCGAGATTCTGACATAACAACAGGCA-3'

Other clones with same target sequence:

  1. TRCN0000349542