Construct: shRNA TRCN0000110442
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_030718.2-630s1c1
- DNA Barcode:
- GCTTTCTACGTGAGGTGGATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Abo (80908)
- Gene Description:
- ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase, transferase B, alpha 1-3-galactosyltransferase)
- Transcript:
- RefSeq NM_030718.2 (NON-CURRENT)
- Match location:
- Position 630 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110442)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTTTCTACGTGAGGTGGATT
- Hairpin Sequence:
-
5'-CCGG-GCTTTCTACGTGAGGTGGATT-CTCGAG-AATCCACCTCACGTAGAAAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTTTCTACGTGAGGTGGATTCTCGAGAATCCACCTCACGTAGAAAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTTTCTACGTGAGGTGGATTCTCGAGAATCCACCTCACGTAGAAAGC-3'
Other clones with same target sequence:
(none)
