Construct: shRNA TRCN0000110443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030718.2-934s1c1
DNA Barcode:
GCATGATGAGAGCTATTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abo (80908)
Gene Description:
ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase, transferase B, alpha 1-3-galactosyltransferase)
Transcript:
RefSeq NM_030718.2 (NON-CURRENT)
Match location:
Position 934 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGATGAGAGCTATTTGAA
Hairpin Sequence:
5'-CCGG-GCATGATGAGAGCTATTTGAA-CTCGAG-TTCAAATAGCTCTCATCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGATGAGAGCTATTTGAACTCGAGTTCAAATAGCTCTCATCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGATGAGAGCTATTTGAACTCGAGTTCAAATAGCTCTCATCATGC-3'

Other clones with same target sequence:

(none)