Construct: shRNA TRCN0000110481

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013701.1-696s1c1
DNA Barcode:
CCGTTTATCAATCACTCCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt1a2 (22236)
Gene Description:
UDP glucuronosyltransferase 1 family, polypeptide A2
Transcript:
RefSeq NM_013701.1 (NON-CURRENT)
Match location:
Position 696 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110481)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTTATCAATCACTCCCTA
Hairpin Sequence:
5'-CCGG-CCGTTTATCAATCACTCCCTA-CTCGAG-TAGGGAGTGATTGATAAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTTATCAATCACTCCCTACTCGAGTAGGGAGTGATTGATAAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTTATCAATCACTCCCTACTCGAGTAGGGAGTGATTGATAAACGG-3'

Other clones with same target sequence:

(none)