Construct: shRNA TRCN0000110509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008663.1-1897s1c1
DNA Barcode:
GCCCTTCTTTGTGCGTTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo7a (17921)
Gene Description:
myosin VIIA
Transcript:
RefSeq NM_008663.1 (NON-CURRENT)
Match location:
Position 1897 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTCTTTGTGCGTTGTAT
Hairpin Sequence:
5'-CCGG-GCCCTTCTTTGTGCGTTGTAT-CTCGAG-ATACAACGCACAAAGAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTCTTTGTGCGTTGTATCTCGAGATACAACGCACAAAGAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTCTTTGTGCGTTGTATCTCGAGATACAACGCACAAAGAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000324295