Construct: shRNA TRCN0000110514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025307.1-1489s1c1
DNA Barcode:
CTGCTGTATTATCCTTGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stx3 (20908)
Gene Description:
syntaxin 3
Transcript:
RefSeq NM_001025307.1 (CURRENT)
Match location:
Position 1489 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTGTATTATCCTTGCCAT
Hairpin Sequence:
5'-CCGG-CTGCTGTATTATCCTTGCCAT-CTCGAG-ATGGCAAGGATAATACAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTGTATTATCCTTGCCATCTCGAGATGGCAAGGATAATACAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTGTATTATCCTTGCCATCTCGAGATGGCAAGGATAATACAGCAG-3'

Other clones with same target sequence:

(none)