Construct: shRNA TRCN0000110555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175260.1-6379s1c1
DNA Barcode:
CCTCCACAAGACATGCGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myh10 (77579)
Gene Description:
myosin, heavy polypeptide 10, non-muscle
Transcript:
RefSeq NM_175260.1 (NON-CURRENT)
Match location:
Position 6379 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCACAAGACATGCGTATT
Hairpin Sequence:
5'-CCGG-CCTCCACAAGACATGCGTATT-CTCGAG-AATACGCATGTCTTGTGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCACAAGACATGCGTATTCTCGAGAATACGCATGTCTTGTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCACAAGACATGCGTATTCTCGAGAATACGCATGTCTTGTGGAGG-3'

Other clones with same target sequence:

(none)