Construct: shRNA TRCN0000110565

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023733.2-2204s1c1
DNA Barcode:
GCCTCAACAAAGCAAATCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crot (74114)
Gene Description:
carnitine O-octanoyltransferase
Transcript:
RefSeq NM_023733.2 (NON-CURRENT)
Match location:
Position 2204 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110565)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAACAAAGCAAATCTAT
Hairpin Sequence:
5'-CCGG-GCCTCAACAAAGCAAATCTAT-CTCGAG-ATAGATTTGCTTTGTTGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAACAAAGCAAATCTATCTCGAGATAGATTTGCTTTGTTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAACAAAGCAAATCTATCTCGAGATAGATTTGCTTTGTTGAGGC-3'

Other clones with same target sequence:

  1. TRCN0000325525