Construct: shRNA TRCN0000110567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023733.2-759s1c1
DNA Barcode:
GCGTTTGTCTTTGATGTACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crot (74114)
Gene Description:
carnitine O-octanoyltransferase
Transcript:
RefSeq NM_023733.2 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTTGTCTTTGATGTACTA
Hairpin Sequence:
5'-CCGG-GCGTTTGTCTTTGATGTACTA-CTCGAG-TAGTACATCAAAGACAAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTTGTCTTTGATGTACTACTCGAGTAGTACATCAAAGACAAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTTGTCTTTGATGTACTACTCGAGTAGTACATCAAAGACAAACGC-3'

Other clones with same target sequence:

  1. TRCN0000325523