Construct: shRNA TRCN0000110571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028021.1-2403s1c1
DNA Barcode:
CACCGACATCATAGTGTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myh14 (71960)
Gene Description:
myosin, heavy polypeptide 14
Transcript:
RefSeq NM_028021.1 (NON-CURRENT)
Match location:
Position 2403 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCGACATCATAGTGTCTTT
Hairpin Sequence:
5'-CCGG-CACCGACATCATAGTGTCTTT-CTCGAG-AAAGACACTATGATGTCGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCGACATCATAGTGTCTTTCTCGAGAAAGACACTATGATGTCGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCGACATCATAGTGTCTTTCTCGAGAAAGACACTATGATGTCGGTG-3'

Other clones with same target sequence:

(none)