Construct: shRNA TRCN0000110579

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009222.2-325s1c1
DNA Barcode:
GAACAACTAAATCGCATAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snap23 (20619)
Gene Description:
synaptosomal-associated protein 23
Transcript:
RefSeq NM_009222.2 (NON-CURRENT)
Match location:
Position 325 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110579)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAACTAAATCGCATAGAA
Hairpin Sequence:
5'-CCGG-GAACAACTAAATCGCATAGAA-CTCGAG-TTCTATGCGATTTAGTTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAACTAAATCGCATAGAACTCGAGTTCTATGCGATTTAGTTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAACTAAATCGCATAGAACTCGAGTTCTATGCGATTTAGTTGTTC-3'

Other clones with same target sequence:

  1. TRCN0000325516