Construct: shRNA TRCN0000110599

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013495.1-294s1c1
DNA Barcode:
GCAAAGATCAATCGGACCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpt1a (12894)
Gene Description:
carnitine palmitoyltransferase 1a, liver
Transcript:
RefSeq NM_013495.1 (NON-CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110599)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGATCAATCGGACCCTA
Hairpin Sequence:
5'-CCGG-GCAAAGATCAATCGGACCCTA-CTCGAG-TAGGGTCCGATTGATCTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGATCAATCGGACCCTACTCGAGTAGGGTCCGATTGATCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGATCAATCGGACCCTACTCGAGTAGGGTCCGATTGATCTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000325519