Construct: shRNA TRCN0000110681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025994.2-346s1c1
DNA Barcode:
GAGAAGATGTTCAAGCAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efhd2 (27984)
Gene Description:
EF hand domain containing 2
Transcript:
RefSeq NM_025994.2 (NON-CURRENT)
Match location:
Position 346 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGATGTTCAAGCAGTAT
Hairpin Sequence:
5'-CCGG-GAGAAGATGTTCAAGCAGTAT-CTCGAG-ATACTGCTTGAACATCTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGATGTTCAAGCAGTATCTCGAGATACTGCTTGAACATCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGATGTTCAAGCAGTATCTCGAGATACTGCTTGAACATCTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000147326