Construct: shRNA TRCN0000110738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011607.1-5991s1c1
DNA Barcode:
CCTGTCCTACAAAGGAGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnc (21923)
Gene Description:
tenascin C
Transcript:
RefSeq NM_011607.1 (NON-CURRENT)
Match location:
Position 5991 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCCTACAAAGGAGCTTT
Hairpin Sequence:
5'-CCGG-CCTGTCCTACAAAGGAGCTTT-CTCGAG-AAAGCTCCTTTGTAGGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCCTACAAAGGAGCTTTCTCGAGAAAGCTCCTTTGTAGGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCCTACAAAGGAGCTTTCTCGAGAAAGCTCCTTTGTAGGACAGG-3'

Other clones with same target sequence:

(none)