Construct: shRNA TRCN0000110768

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031176.1-9444s1c1
DNA Barcode:
CGACTCTTTCTTAGTCCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnxb (81877)
Gene Description:
tenascin XB
Transcript:
RefSeq NM_031176.1 (NON-CURRENT)
Match location:
Position 9444 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110768)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTCTTTCTTAGTCCAGTA
Hairpin Sequence:
5'-CCGG-CGACTCTTTCTTAGTCCAGTA-CTCGAG-TACTGGACTAAGAAAGAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTCTTTCTTAGTCCAGTACTCGAGTACTGGACTAAGAAAGAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTCTTTCTTAGTCCAGTACTCGAGTACTGGACTAAGAAAGAGTCG-3'

Other clones with same target sequence:

(none)