Construct: shRNA TRCN0000110797

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033521.2-604s1c1
DNA Barcode:
CCCGTTCTTCTGTTACCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Laptm4b (114128)
Gene Description:
lysosomal-associated protein transmembrane 4B
Transcript:
RefSeq NM_033521.2 (NON-CURRENT)
Match location:
Position 604 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110797)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTTCTTCTGTTACCAGAT
Hairpin Sequence:
5'-CCGG-CCCGTTCTTCTGTTACCAGAT-CTCGAG-ATCTGGTAACAGAAGAACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTTCTTCTGTTACCAGATCTCGAGATCTGGTAACAGAAGAACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTTCTTCTGTTACCAGATCTCGAGATCTGGTAACAGAAGAACGGG-3'

Other clones with same target sequence:

  1. TRCN0000325500