Construct: shRNA TRCN0000110808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011920.2-1554s1c1
DNA Barcode:
GCCAGTCTATGTTACCTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcg2 (26357)
Gene Description:
ATP-binding cassette, sub-family G (WHITE), member 2
Transcript:
RefSeq NM_011920.2 (NON-CURRENT)
Match location:
Position 1554 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTCTATGTTACCTCTTT
Hairpin Sequence:
5'-CCGG-GCCAGTCTATGTTACCTCTTT-CTCGAG-AAAGAGGTAACATAGACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTCTATGTTACCTCTTTCTCGAGAAAGAGGTAACATAGACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTCTATGTTACCTCTTTCTCGAGAAAGAGGTAACATAGACTGGC-3'

Other clones with same target sequence:

  1. TRCN0000325480