Construct: shRNA TRCN0000110821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009980.1-170s1c1
DNA Barcode:
CCAGCAGCAATATGGCTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc10a7 (76775)
Gene Description:
solute carrier family 10 (sodium/bile acid cotransporter family), member 7
Transcript:
RefSeq NM_001009980.1 (NON-CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAGCAATATGGCTCTTT
Hairpin Sequence:
5'-CCGG-CCAGCAGCAATATGGCTCTTT-CTCGAG-AAAGAGCCATATTGCTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAGCAATATGGCTCTTTCTCGAGAAAGAGCCATATTGCTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAGCAATATGGCTCTTTCTCGAGAAAGAGCCATATTGCTGCTGG-3'

Other clones with same target sequence:

(none)