Construct: shRNA TRCN0000110839

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173774.2-960s1c1
DNA Barcode:
GCTCCGTGTCATCTATGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc45a1 (242773)
Gene Description:
solute carrier family 45, member 1
Transcript:
RefSeq NM_173774.2 (NON-CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110839)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCGTGTCATCTATGTCTT
Hairpin Sequence:
5'-CCGG-GCTCCGTGTCATCTATGTCTT-CTCGAG-AAGACATAGATGACACGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCGTGTCATCTATGTCTTCTCGAGAAGACATAGATGACACGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCGTGTCATCTATGTCTTCTCGAGAAGACATAGATGACACGGAGC-3'

Other clones with same target sequence:

(none)