Construct: shRNA TRCN0000110870
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_030239.2-2584s1c1
- DNA Barcode:
- GCAGTCATTGATTCACATTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Abcg3 (27405)
- Gene Description:
- ATP-binding cassette, sub-family G (WHITE), member 3
- Transcript:
- RefSeq NM_030239.2 (CURRENT)
- Match location:
- Position 2584 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110870)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGTCATTGATTCACATTTA
- Hairpin Sequence:
-
5'-CCGG-GCAGTCATTGATTCACATTTA-CTCGAG-TAAATGTGAATCAATGACTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGTCATTGATTCACATTTACTCGAGTAAATGTGAATCAATGACTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGTCATTGATTCACATTTACTCGAGTAAATGTGAATCAATGACTGC-3'
Other clones with same target sequence:
(none)
