Construct: shRNA TRCN0000111043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176839.1-804s1c1
DNA Barcode:
GCTGTATTCTGCGGTCAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcc5 (27416)
Gene Description:
ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Transcript:
RefSeq NM_176839.1 (CURRENT)
Match location:
Position 804 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTATTCTGCGGTCAGAAT
Hairpin Sequence:
5'-CCGG-GCTGTATTCTGCGGTCAGAAT-CTCGAG-ATTCTGACCGCAGAATACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTATTCTGCGGTCAGAATCTCGAGATTCTGACCGCAGAATACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTATTCTGCGGTCAGAATCTCGAGATTCTGACCGCAGAATACAGC-3'

Other clones with same target sequence:

(none)