Construct: shRNA TRCN0000111060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008576.2-5428s1c1
DNA Barcode:
CGGGCAAGAATCTGGTATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcc1 (17250)
Gene Description:
ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Transcript:
RefSeq NM_008576.2 (NON-CURRENT)
Match location:
Position 5428 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCAAGAATCTGGTATCAA
Hairpin Sequence:
5'-CCGG-CGGGCAAGAATCTGGTATCAA-CTCGAG-TTGATACCAGATTCTTGCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCAAGAATCTGGTATCAACTCGAGTTGATACCAGATTCTTGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCAAGAATCTGGTATCAACTCGAGTTGATACCAGATTCTTGCCCG-3'

Other clones with same target sequence:

(none)