Construct: shRNA TRCN0000111138
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_127411.3-846s1c1
- DNA Barcode:
- CCTTGCAGAAATGGCGGAATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Edil3 (13612)
- Gene Description:
- EGF-like repeats and discoidin I-like domains 3
- Transcript:
- RefSeq XM_127411.3 (NON-CURRENT)
- Match location:
- Position 846 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000111138)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTTGCAGAAATGGCGGAATA
- Hairpin Sequence:
-
5'-CCGG-CCTTGCAGAAATGGCGGAATA-CTCGAG-TATTCCGCCATTTCTGCAAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTTGCAGAAATGGCGGAATACTCGAGTATTCCGCCATTTCTGCAAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTTGCAGAAATGGCGGAATACTCGAGTATTCCGCCATTTCTGCAAGG-3'
Other clones with same target sequence:
-
TRCN0000317863
