Construct: shRNA TRCN0000111139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127411.3-760s1c1
DNA Barcode:
ACACATTCATAGGCTATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Edil3 (13612)
Gene Description:
EGF-like repeats and discoidin I-like domains 3
Transcript:
RefSeq XM_127411.3 (NON-CURRENT)
Match location:
Position 760 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACATTCATAGGCTATGTTT
Hairpin Sequence:
5'-CCGG-ACACATTCATAGGCTATGTTT-CTCGAG-AAACATAGCCTATGAATGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACATTCATAGGCTATGTTTCTCGAGAAACATAGCCTATGAATGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACATTCATAGGCTATGTTTCTCGAGAAACATAGCCTATGAATGTGT-3'

Other clones with same target sequence:

  1. TRCN0000317803