Construct: shRNA TRCN0000111170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019636.1-3848s1c1
DNA Barcode:
GAGGCAGAGAAGAGAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d1 (57915)
Gene Description:
TBC1 domain family, member 1
Transcript:
RefSeq NM_019636.1 (NON-CURRENT)
Match location:
Position 3848 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCAGAGAAGAGAGAACTT
Hairpin Sequence:
5'-CCGG-GAGGCAGAGAAGAGAGAACTT-CTCGAG-AAGTTCTCTCTTCTCTGCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCAGAGAAGAGAGAACTTCTCGAGAAGTTCTCTCTTCTCTGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCAGAGAAGAGAGAACTTCTCGAGAAGTTCTCTCTTCTCTGCCTC-3'

Other clones with same target sequence:

(none)