Construct: shRNA TRCN0000111255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008325.1-2523s1c1
DNA Barcode:
GCACAGAGTAAGCATCTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Idua (15932)
Gene Description:
iduronidase, alpha-L-
Transcript:
RefSeq NM_008325.1 (NON-CURRENT)
Match location:
Position 2523 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGAGTAAGCATCTTCAT
Hairpin Sequence:
5'-CCGG-GCACAGAGTAAGCATCTTCAT-CTCGAG-ATGAAGATGCTTACTCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGAGTAAGCATCTTCATCTCGAGATGAAGATGCTTACTCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGAGTAAGCATCTTCATCTCGAGATGAAGATGCTTACTCTGTGC-3'

Other clones with same target sequence:

(none)