Construct: shRNA TRCN0000111346

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177546.2-803s1c1
DNA Barcode:
GCATGTTTGTTCCAACACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcyt1b (236899)
Gene Description:
phosphate cytidylyltransferase 1, choline, beta isoform
Transcript:
RefSeq NM_177546.2 (CURRENT)
Match location:
Position 803 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111346)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTTTGTTCCAACACAAA
Hairpin Sequence:
5'-CCGG-GCATGTTTGTTCCAACACAAA-CTCGAG-TTTGTGTTGGAACAAACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTTTGTTCCAACACAAACTCGAGTTTGTGTTGGAACAAACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTTTGTTCCAACACAAACTCGAGTTTGTGTTGGAACAAACATGC-3'

Other clones with same target sequence:

(none)