Construct: shRNA TRCN0000111370
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011293.1-372s1c1
- DNA Barcode:
- AGCTGAAAGAAGCGTTGCTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Polr2j (20022)
- Gene Description:
- polymerase (RNA) II (DNA directed) polypeptide J
- Transcript:
- RefSeq NM_011293.1 (NON-CURRENT)
- Match location:
- Position 372 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000111370)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGCTGAAAGAAGCGTTGCTTA
- Hairpin Sequence:
-
5'-CCGG-AGCTGAAAGAAGCGTTGCTTA-CTCGAG-TAAGCAACGCTTCTTTCAGCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGCTGAAAGAAGCGTTGCTTACTCGAGTAAGCAACGCTTCTTTCAGCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGCTGAAAGAAGCGTTGCTTACTCGAGTAAGCAACGCTTCTTTCAGCT-3'
Other clones with same target sequence:
-
TRCN0000332516
