Construct: shRNA TRCN0000111370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011293.1-372s1c1
DNA Barcode:
AGCTGAAAGAAGCGTTGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Polr2j (20022)
Gene Description:
polymerase (RNA) II (DNA directed) polypeptide J
Transcript:
RefSeq NM_011293.1 (NON-CURRENT)
Match location:
Position 372 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGAAAGAAGCGTTGCTTA
Hairpin Sequence:
5'-CCGG-AGCTGAAAGAAGCGTTGCTTA-CTCGAG-TAAGCAACGCTTCTTTCAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGAAAGAAGCGTTGCTTACTCGAGTAAGCAACGCTTCTTTCAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGAAAGAAGCGTTGCTTACTCGAGTAAGCAACGCTTCTTTCAGCT-3'

Other clones with same target sequence:

  1. TRCN0000332516