Construct: shRNA TRCN0000111429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009086.1-2821s1c1
DNA Barcode:
CCGTATGACCATAGGTATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Polr1b (20017)
Gene Description:
polymerase (RNA) I polypeptide B
Transcript:
RefSeq NM_009086.1 (NON-CURRENT)
Match location:
Position 2821 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTATGACCATAGGTATGTT
Hairpin Sequence:
5'-CCGG-CCGTATGACCATAGGTATGTT-CTCGAG-AACATACCTATGGTCATACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTATGACCATAGGTATGTTCTCGAGAACATACCTATGGTCATACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTATGACCATAGGTATGTTCTCGAGAACATACCTATGGTCATACGG-3'

Other clones with same target sequence:

  1. TRCN0000326604