Construct: shRNA TRCN0000111459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020032.1-1205s1c1
DNA Barcode:
CTTGAAGCACTACGATGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Poll (56626)
Gene Description:
polymerase (DNA directed), lambda
Transcript:
RefSeq NM_020032.1 (NON-CURRENT)
Match location:
Position 1205 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGAAGCACTACGATGACTT
Hairpin Sequence:
5'-CCGG-CTTGAAGCACTACGATGACTT-CTCGAG-AAGTCATCGTAGTGCTTCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGAAGCACTACGATGACTTCTCGAGAAGTCATCGTAGTGCTTCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGAAGCACTACGATGACTTCTCGAGAAGTCATCGTAGTGCTTCAAG-3'

Other clones with same target sequence:

  1. TRCN0000288287