Construct: shRNA TRCN0000111538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028807.2-450s1c1
DNA Barcode:
CCTGAGAACTTCAACCCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Exoc3l4 (74190)
Gene Description:
exocyst complex component 3-like 4
Transcript:
RefSeq NM_028807.2 (NON-CURRENT)
Match location:
Position 450 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGAACTTCAACCCACAA
Hairpin Sequence:
5'-CCGG-CCTGAGAACTTCAACCCACAA-CTCGAG-TTGTGGGTTGAAGTTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGAACTTCAACCCACAACTCGAGTTGTGGGTTGAAGTTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGAACTTCAACCCACAACTCGAGTTGTGGGTTGAAGTTCTCAGG-3'

Other clones with same target sequence:

(none)