Construct: shRNA TRCN0000111555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022997.2-2710s1c1
DNA Barcode:
CCACTTATTCTGGCTACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps35 (65114)
Gene Description:
VPS35 retromer complex component
Transcript:
RefSeq NM_022997.2 (NON-CURRENT)
Match location:
Position 2710 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTATTCTGGCTACTAAT
Hairpin Sequence:
5'-CCGG-CCACTTATTCTGGCTACTAAT-CTCGAG-ATTAGTAGCCAGAATAAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTATTCTGGCTACTAATCTCGAGATTAGTAGCCAGAATAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTATTCTGGCTACTAATCTCGAGATTAGTAGCCAGAATAAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000318222