Construct: shRNA TRCN0000111568

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010587.1-1547s1c1
DNA Barcode:
GAGAGCTAAGAATTGCTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itsn1 (16443)
Gene Description:
intersectin 1 (SH3 domain protein 1A)
Transcript:
RefSeq NM_010587.1 (NON-CURRENT)
Match location:
Position 1547 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111568)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGCTAAGAATTGCTGAAA
Hairpin Sequence:
5'-CCGG-GAGAGCTAAGAATTGCTGAAA-CTCGAG-TTTCAGCAATTCTTAGCTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGCTAAGAATTGCTGAAACTCGAGTTTCAGCAATTCTTAGCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGCTAAGAATTGCTGAAACTCGAGTTTCAGCAATTCTTAGCTCTC-3'

Other clones with same target sequence:

(none)