Construct: shRNA TRCN0000111642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021305.2-1162s1c1
DNA Barcode:
CCTGTTCATGTAGTTGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec61a2 (57743)
Gene Description:
Sec61, alpha subunit 2 (S. cerevisiae)
Transcript:
RefSeq NM_021305.2 (NON-CURRENT)
Match location:
Position 1162 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTCATGTAGTTGTATAT
Hairpin Sequence:
5'-CCGG-CCTGTTCATGTAGTTGTATAT-CTCGAG-ATATACAACTACATGAACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTCATGTAGTTGTATATCTCGAGATATACAACTACATGAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTCATGTAGTTGTATATCTCGAGATATACAACTACATGAACAGG-3'

Other clones with same target sequence:

  1. TRCN0000309861