Construct: shRNA TRCN0000111648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172882.2-10613s1c1
DNA Barcode:
GCGCAGTTACAGCAGATTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdfy3 (72145)
Gene Description:
WD repeat and FYVE domain containing 3
Transcript:
RefSeq NM_172882.2 (NON-CURRENT)
Match location:
Position 10613 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAGTTACAGCAGATTGAA
Hairpin Sequence:
5'-CCGG-GCGCAGTTACAGCAGATTGAA-CTCGAG-TTCAATCTGCTGTAACTGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAGTTACAGCAGATTGAACTCGAGTTCAATCTGCTGTAACTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAGTTACAGCAGATTGAACTCGAGTTCAATCTGCTGTAACTGCGC-3'

Other clones with same target sequence:

  1. TRCN0000351338