Construct: shRNA TRCN0000111658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019490.1-2512s1c1
DNA Barcode:
CGAGCAAGTTGCAGAGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uso1 (56041)
Gene Description:
USO1 vesicle docking factor
Transcript:
RefSeq NM_019490.1 (CURRENT)
Match location:
Position 2512 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCAAGTTGCAGAGTTAAA
Hairpin Sequence:
5'-CCGG-CGAGCAAGTTGCAGAGTTAAA-CTCGAG-TTTAACTCTGCAACTTGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCAAGTTGCAGAGTTAAACTCGAGTTTAACTCTGCAACTTGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCAAGTTGCAGAGTTAAACTCGAGTTTAACTCTGCAACTTGCTCG-3'

Other clones with same target sequence:

  1. TRCN0000309866