Construct: shRNA TRCN0000111664

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018829.2-127s1c1
DNA Barcode:
CCAGTCAGTGTGTGACTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap3m1 (55946)
Gene Description:
adaptor-related protein complex 3, mu 1 subunit
Transcript:
RefSeq NM_018829.2 (NON-CURRENT)
Match location:
Position 127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111664)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCAGTGTGTGACTATTT
Hairpin Sequence:
5'-CCGG-CCAGTCAGTGTGTGACTATTT-CTCGAG-AAATAGTCACACACTGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCAGTGTGTGACTATTTCTCGAGAAATAGTCACACACTGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCAGTGTGTGACTATTTCTCGAGAAATAGTCACACACTGACTGG-3'

Other clones with same target sequence:

  1. TRCN0000309922