Construct: shRNA TRCN0000111716

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025828.2-838s1c1
DNA Barcode:
CCTTCCTAGCTGTACGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lman2 (66890)
Gene Description:
lectin, mannose-binding 2
Transcript:
RefSeq NM_025828.2 (NON-CURRENT)
Match location:
Position 838 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111716)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCTAGCTGTACGCTATT
Hairpin Sequence:
5'-CCGG-CCTTCCTAGCTGTACGCTATT-CTCGAG-AATAGCGTACAGCTAGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCTAGCTGTACGCTATTCTCGAGAATAGCGTACAGCTAGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCTAGCTGTACGCTATTCTCGAGAATAGCGTACAGCTAGGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000334067