Construct: shRNA TRCN0000111723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007943.1-2485s1c1
DNA Barcode:
CCATCAACAAATTGGATTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eps15 (13858)
Gene Description:
epidermal growth factor receptor pathway substrate 15
Transcript:
RefSeq NM_007943.1 (NON-CURRENT)
Match location:
Position 2485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAACAAATTGGATTCTT
Hairpin Sequence:
5'-CCGG-CCATCAACAAATTGGATTCTT-CTCGAG-AAGAATCCAATTTGTTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAACAAATTGGATTCTTCTCGAGAAGAATCCAATTTGTTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAACAAATTGGATTCTTCTCGAGAAGAATCCAATTTGTTGATGG-3'

Other clones with same target sequence:

(none)