Construct: shRNA TRCN0000111730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133255.1-2335s1c1
DNA Barcode:
GCTTCCTTAGATGGGAGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hook2 (170833)
Gene Description:
hook microtubule tethering protein 2
Transcript:
RefSeq NM_133255.1 (NON-CURRENT)
Match location:
Position 2335 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCTTAGATGGGAGAGAA
Hairpin Sequence:
5'-CCGG-GCTTCCTTAGATGGGAGAGAA-CTCGAG-TTCTCTCCCATCTAAGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCTTAGATGGGAGAGAACTCGAGTTCTCTCCCATCTAAGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCTTAGATGGGAGAGAACTCGAGTTCTCTCCCATCTAAGGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000316232