Construct: shRNA TRCN0000111747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009679.1-972s1c1
DNA Barcode:
CCGTACTACCAAGGACATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap2m1 (11773)
Gene Description:
adaptor-related protein complex 2, mu 1 subunit
Transcript:
RefSeq NM_009679.1 (NON-CURRENT)
Match location:
Position 972 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTACTACCAAGGACATCAT
Hairpin Sequence:
5'-CCGG-CCGTACTACCAAGGACATCAT-CTCGAG-ATGATGTCCTTGGTAGTACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTACTACCAAGGACATCATCTCGAGATGATGTCCTTGGTAGTACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTACTACCAAGGACATCATCTCGAGATGATGTCCTTGGTAGTACGG-3'

Other clones with same target sequence:

(none)