Construct: shRNA TRCN0000111780

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146129.2-2531s1c1
DNA Barcode:
GCCAGGGACATAGGAATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcif1 (228866)
Gene Description:
PDX1 C-terminal inhibiting factor 1
Transcript:
RefSeq NM_146129.2 (NON-CURRENT)
Match location:
Position 2531 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111780)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGGACATAGGAATATTA
Hairpin Sequence:
5'-CCGG-GCCAGGGACATAGGAATATTA-CTCGAG-TAATATTCCTATGTCCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGGACATAGGAATATTACTCGAGTAATATTCCTATGTCCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGGACATAGGAATATTACTCGAGTAATATTCCTATGTCCCTGGC-3'

Other clones with same target sequence:

(none)