Construct: shRNA TRCN0000111794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028295.1-780s1c1
DNA Barcode:
GCTACCCTACCATCTGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdia5 (72599)
Gene Description:
protein disulfide isomerase associated 5
Transcript:
RefSeq NM_028295.1 (CURRENT)
Match location:
Position 780 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACCCTACCATCTGCTATT
Hairpin Sequence:
5'-CCGG-GCTACCCTACCATCTGCTATT-CTCGAG-AATAGCAGATGGTAGGGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACCCTACCATCTGCTATTCTCGAGAATAGCAGATGGTAGGGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACCCTACCATCTGCTATTCTCGAGAATAGCAGATGGTAGGGTAGC-3'

Other clones with same target sequence:

  1. TRCN0000332797