Construct: shRNA TRCN0000111861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010219.2-781s1c1
DNA Barcode:
CCTCAAACCTAGCTATGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fkbp4 (14228)
Gene Description:
FK506 binding protein 4
Transcript:
RefSeq NM_010219.2 (NON-CURRENT)
Match location:
Position 781 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAACCTAGCTATGCTTT
Hairpin Sequence:
5'-CCGG-CCTCAAACCTAGCTATGCTTT-CTCGAG-AAAGCATAGCTAGGTTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAACCTAGCTATGCTTTCTCGAGAAAGCATAGCTAGGTTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAACCTAGCTATGCTTTCTCGAGAAAGCATAGCTAGGTTTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000351875