Construct: shRNA TRCN0000111896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172424.2-4282s1c1
DNA Barcode:
GCCACCGTGATGTTGCTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med13l (76199)
Gene Description:
mediator complex subunit 13-like
Transcript:
RefSeq NM_172424.2 (NON-CURRENT)
Match location:
Position 4282 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCGTGATGTTGCTTATA
Hairpin Sequence:
5'-CCGG-GCCACCGTGATGTTGCTTATA-CTCGAG-TATAAGCAACATCACGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCGTGATGTTGCTTATACTCGAGTATAAGCAACATCACGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCGTGATGTTGCTTATACTCGAGTATAAGCAACATCACGGTGGC-3'

Other clones with same target sequence:

(none)