Construct: shRNA TRCN0000111918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175367.2-1485s1c1
DNA Barcode:
CTTTCTTTGAAGAGCAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ston2 (108800)
Gene Description:
stonin 2
Transcript:
RefSeq NM_175367.2 (NON-CURRENT)
Match location:
Position 1485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTCTTTGAAGAGCAGGAAA
Hairpin Sequence:
5'-CCGG-CTTTCTTTGAAGAGCAGGAAA-CTCGAG-TTTCCTGCTCTTCAAAGAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTCTTTGAAGAGCAGGAAACTCGAGTTTCCTGCTCTTCAAAGAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTCTTTGAAGAGCAGGAAACTCGAGTTTCCTGCTCTTCAAAGAAAG-3'

Other clones with same target sequence:

(none)