Construct: shRNA TRCN0000111959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194262.1-2521s1c1
DNA Barcode:
GCAGAAATGAACTGATTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arid4b (94246)
Gene Description:
AT rich interactive domain 4B (RBP1-like)
Transcript:
RefSeq NM_194262.1 (NON-CURRENT)
Match location:
Position 2521 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAATGAACTGATTTCAA
Hairpin Sequence:
5'-CCGG-GCAGAAATGAACTGATTTCAA-CTCGAG-TTGAAATCAGTTCATTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAATGAACTGATTTCAACTCGAGTTGAAATCAGTTCATTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAATGAACTGATTTCAACTCGAGTTGAAATCAGTTCATTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000341006