Construct: shRNA TRCN0000112043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178631.2-1518s1c1
DNA Barcode:
CGATTACTACAGAGATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ralyl (76897)
Gene Description:
RALY RNA binding protein-like
Transcript:
RefSeq NM_178631.2 (NON-CURRENT)
Match location:
Position 1518 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATTACTACAGAGATGATTT
Hairpin Sequence:
5'-CCGG-CGATTACTACAGAGATGATTT-CTCGAG-AAATCATCTCTGTAGTAATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATTACTACAGAGATGATTTCTCGAGAAATCATCTCTGTAGTAATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATTACTACAGAGATGATTTCTCGAGAAATCATCTCTGTAGTAATCG-3'

Other clones with same target sequence:

(none)