Construct: shRNA TRCN0000112078

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028871.1-1593s1c1
DNA Barcode:
GAAGATCCCTACTACGGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hnrnpr (74326)
Gene Description:
heterogeneous nuclear ribonucleoprotein R
Transcript:
RefSeq NM_028871.1 (NON-CURRENT)
Match location:
Position 1593 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112078)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATCCCTACTACGGCTAT
Hairpin Sequence:
5'-CCGG-GAAGATCCCTACTACGGCTAT-CTCGAG-ATAGCCGTAGTAGGGATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATCCCTACTACGGCTATCTCGAGATAGCCGTAGTAGGGATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATCCCTACTACGGCTATCTCGAGATAGCCGTAGTAGGGATCTTC-3'

Other clones with same target sequence:

  1. TRCN0000308914